Applikon Mini Bioreators

Applikon Mini Bioreators

The MiniBio range of bioreactors (250 ml, 500 ml and 1000 ml total volume) is a true scale down of the laboratory scale bioreactors in the 1 to 15 liter range. The MiniBio systems have the same flexibility as the laboratory scale bioreactors. This means that the MiniBio systems can be customized to fit the demands of any process. The small volume reduces medium costs and reduces the use expensive bench space.

Functions of mini bioreactors:

  • Generate more data in less time;
  • Easy setup and operation;
  • Cultivate using less medium;
  • Cultivate using les bench space;
  • Generate scalable results;
  • Mimic lab scale bioreactors;
  • Easy data handling.

Features of the miniBio:

  • Generate more data in less time
  • Easy setup and operation
  • Cultivate using less medium
  • Cultivate using less bench space
  • Generate scalable results
    • Screening studies
    • Media optimization
    • Process optimization
    • Microbial and Cell culture
  • Batch, Fed-Batch, Perfusion and Continuous cultivation


MiniBio 250 MiniBio 500 MiniBio 1000
Total volume (ml) 250 500 1000
Working volume (ml) 200 400 800
Minimum working volume (ml) 50 100 200
Aspect ratio total volume 2.5



Aspect ratio working volume 2 2 2
Dimensions (dxh, mm)  180 x 400  195 x 400  200 x 550
Dimensions for autoclaving (dxh, mm)  180 x 250 195 x 250  200 x 400
Drive system Direct drive, lipsealed Direct drive, lipsealed Direct drive, lipsealed
Maximum stirrerspeed (rpm) 50 – 2000 50 - 1750 50 - 1500
Maximum impeller tipspeed (m/s)  2.3 2.0 3.5
Impellers Choice of Rushton and marine
Gas sparger Porous sparger or L-type sparger
Gas overlay Yes
Exhaust gas Electrically cooled exhaust gas condenser
(evaporation <5% per day at 37°C @ 2vvm)
Sampling Fixed sample pipe with optional sampling system
Draining Height adjustable drain pipe
Additions 4 fixed inlet ports and optional micro liquid injectors
pH Measurement: 8 mm classic pH sensor
Control: via acid pump (variable speed pump)or CO2 gas in combination with alkali pump (variable speed pump)
DO2 Measurement: 8 mm classic polarographic DO2 sensor
Control: via a combination of N2, Air, O2  (optional MFC) and agitation or nutrient addition (variable speed pump)
Temperature Measurement: Pt-100 sensor in thermowell in topplate
Control: electrical cooling and heating jacket via bioreactor wall
Foam Measurement: Height adjustable conductivity based foam sensor
Control: Anti foam addition (variable speed pump)
Level Measurement: Height adjustable conductivity based level sensor
Control: variable speed pump for liquid addition or removal
Optional inlets Septum, chemostat tube, liquid entry system



my-Control is a dedicated control system for the small scale bioreactors. One control system can control:

  • 250 ml up to 1000 ml MiniBio bioreactors;
  • 1l up to 3 liter Autoclavable bioreactors;
  • 3 liter Cellready single use bioreactors.

Mini Systems - my-Control and MiniBio Catalogue