IntroductionIn the laboratory bioreactor and fermentor range Applikon is worldwide market leader because of its dependable and easy to use systems. The bioreactors and fermentors excel in quality and modularity. An Applikon laboratory system is easy to upgrade if a change in research activities occurs. Applikon offers glass autoclavable bioreactors for cell culture applications and glass autoclavable fermentors for microbial culture applications. The systems are built according to the specific demands of a process using an extensive array of standard components. Because of the modularity and flexibility, the user can always adapt the systems to changed process demands. This results in low initial investment and low running costs. The stirred tank reactor (STR) is the most widely used bioreactor type. Glass autoclavable bioreactors and fermentors are available in 1 - 2 - 3 - 5 - 7 - 15 and 20 liter total volume.
Specifications
Autoclavable bioreactor specifications:
Total volume (liter) |
Working volume (liter) |
Minimum working volume (liter) |
Aspect ratio total volume |
Aspect ratio working volume |
|
1 liter |
1 |
0.9 |
0.3 |
1.9 |
1.5 |
2 liter single wall 2 liter jacketed |
2 2 |
1.7 1.7 |
0.5 0.5 |
2.2 |
1.9 1.9 |
3 liter single wall 3 liter jacketed |
3 3 |
2.7 2.7 |
0.5 0.5 |
1.7 |
1.5 1.5 |
5 liter single wall 5 liter jacketed |
5 5 |
3.4 3.4 |
2.0 2.0 |
1.6 |
1.1 1.1 |
7 liter single wall 7 liter jacketed |
7 7 |
5.4 5.4 |
2.0 2.0 |
2.3 |
1.8 1.8 |
15 liter single wall 15 liter jacketed |
15 15 |
12 12 |
2.5 2.5 |
1.9 |
1.5 1.5 |
20 liter single wall |
20 |
16 |
2.5 |
2.5 |
2.0 |
Drive system |
Direct drive, lipsealed or magnetically coupled |
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Maximum stirrerspeed (rpm) |
Standard range is 50 - 1250. 1, 2 and 3 liter systems can be supplied with 2000 rpm motor |
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Impellers |
Rushton and marine with outside diameters 45mm, 60 mm 75 mm or 85 mm |
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Gas sparger |
Porous sparger or L-type sparger |
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Gas overlay |
Yes |
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Exhaust gas |
Water cooled exhaust gas condenser |
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Sampling |
Fixed height or height adjustable sample pipe with optional sampling system Sample pipe internal diameters choices are: 1.7 mm, 4 mm, 6 mm or 10 mm |
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Draining |
Drain pipe |
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Additions |
Triple or single inlet ports and optional micro liquid injectors |
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pH |
Measurement: 12 mm classic pH sensor Control: via acid pump or CO2 gas (rotameter or MFC) in combination with alkali pump |
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DO2 |
Measurement: 12 mm classic polarographic DO2 sensor Control: via a combination of N2, Air, O2 (Rotameter or MFC) and agitation or nutrient addition pump |
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Temperature |
Measurement: Pt-100 sensor in thermowell in topplate Control: cooling and/or heating jacket via bioreactor wall or via internal heat exchanger |
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Foam |
Measurement: Height adjustable conductivity based foam sensor Control: Anti foam addition pump |
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Level |
Measurement: Height adjustable conductivity based level sensor Control: pump for liquid addition or removal |
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Optional inlets |
Septum, chemostat tube, liquid entry system |
Applications
Microbial and Cell culture configuration
Batch, Fed-Batch, Perfusion and Continuous cultivation
Features of the glass autoclavable bioreactors:
RTD Technology Ltd T/A Asistec
Unit 23, Southern Cross Business Park
Boghall Road, Bray
Co. Wicklow, A98 XC52
Tel: +353 (0)1 286 8796
Tel: +353 (0)1 286 8797
Fax: +353 (0)1 286 5380